Pengaruh Ekstrak Daun Buncis (Phaseolus vulgaris L.) Terhadap Uji Antioksidan Dan Anti Aging Tikus Galur Wistar Yang Terpapar Sinar UVB

Oky Pratama, Trishna Trishna, Widyaningsih Oentari

Abstract


Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun buncis (Phaseolus vulgaris L.) terhadap aktivitas antioksidan dan anti-aging pada tikus galur Wistar yang terpapar sinar UVB. Paparan sinar UVB dapat menyebabkan stres oksidatif dan penuaan dini dengan meningkatkan produksi radikal bebas pada kulit. Dalam penelitian ini, tikus diberi perlakuan dengan dosis ekstrak daun buncis yang berbeda, kemudian dilakukan pengukuran aktivitas antioksidan melalui uji biokimia. Efek terhadap tanda-tanda penuaan juga dianalisis melalui pemeriksaan histologi kulit dan kadar kolagen. Hasil penelitian menunjukkan bahwa ekstrak daun buncis secara signifikan meningkatkan aktivitas antioksidan dan mengurangi tanda-tanda penuaan pada kulit tikus yang terpapar sinar UVB. Temuan ini menunjukkan bahwa ekstrak daun buncis berpotensi sebagai agen alami untuk mencegah stres oksidatif dan penuaan kulit yang disebabkan oleh paparan sinar UVB.

Keywords


Phaseolus vulgaris L.; Antioksidan; Anti-Aging; Sinar UVB; Tikus Galur Wistar.

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References


Abe, Y., & Nishizawa, M. (2021). Electrical aspects of skin as a pathway to engineering skin devices. APL Bioengineering, 5(4).

Alamzeb, M., Khan, B., Ullah, I., Omer, M., & Adnan. (2024). Natural antioxidants: An update. IntechOpen. https://doi.org/10.5772/intechopen.112462

Amorati, R., & Valgimigli, L. (2018). Methods to measure the antioxidant activity of phytochemicals and plant extracts. Journal of Agricultural and Food Chemistry, 66(13), 3324–3329.

Andarwati, A. S. (2017). Penetapan kadar vitamin C pada kacang buncis (Phaseolus vulgaris L.) segar dan rebus secara spektrofotometri UV [Doctoral dissertation, Universitas Setia Budi].

Ansary, T. M., Hossain, M. R., Kamiya, K., Komine, M., & Ohtsuki, M. (2021). Inflammatory molecules associated with ultraviolet radiation mediated skin aging. International Journal of Molecular Sciences, 22(8), 3974.

Cahyadi, W. (2016). Analisis dan aspek kesehatan bahan tambahan pangan. Jakarta, Indonesia: PT Bumi Aksara.

Cahyono. (2003). Teknik budidaya dan analisis usaha tani. Yogyakarta, Indonesia: Kanisius.

Cahyono. (2017). Kacang buncis, teknik budidaya dan analisis usaha tani. Yogyakarta, Indonesia: Kanisius.

Cirmi, S., Maugeri, A., Ferlazzo, N., et al. (2017). Anticancer potential of citrus juices and their extracts: A systematic review of both preclinical and clinical studies. Frontiers in Pharmacology, 8, 420.

Cortés Rojas, D. F., de Souza, C. R. F., & Oliveira, W. P. (2014). Clove (Syzygium aromaticum): A precious spice. Asian Pacific Journal of Tropical Biomedicine, 4(2), 90–96.

Dalimartha, S. (2005). Tanaman obat di lingkungan sekitar. Jakarta, Indonesia: Puspa Swara.

Deviani, F., Rochdiani, D., & Saefudin, B. R. (2019). Analisis faktor faktor yang mempengaruhi produksi usaha tani buncis di gabungan kelompok tani Lembang Agri Kabupaten Bandung Barat. Agrisocionomics: Jurnal Sosial Ekonomi Pertanian, 3(2), 165–173.

El Ghallab, Y., Al Jahid, A., Jamal Eddine, J., Ait Haj Said, A., Zarayby, L., & Derfoufi, S. (2020). Syzygium aromaticum L.: Phytochemical investigation and comparison of the scavenging activity of essential oil, extracts and eugenol. Advances in Traditional Medicine, 20, 153–158.

Fauzya, A. F., Astuti, R. I., & Mubarik, N. R. (2019). Effect of ethanol derived clove leaf extract on the oxidative stress response in yeast Schizosaccharomyces pombe. International Journal of Microbiology, 2019.

Fuller, B. (2019). Role of PGE 2 and other inflammatory mediators in skin aging and their inhibition by topical natural anti inflammatories. Cosmetics, 6(1), 6.

Ganguly, B., Hota, M., & Pradhan, J. (2022). Skin aging: Implications of UV radiation, reactive oxygen species and natural antioxidants. IntechOpen. https://doi.org/10.5772/intechopen.100102

Gengatharan, A., & Abd Rahim, M. H. (2023). The application of clove extracts as a potential functional component in active food packaging materials and model food systems: A mini review. Applied Food Research, 3(1), 100283.

Gulcin, İ., & Alwasel, S. H. (2023). DPPH radical scavenging assay. Processes, 11(8), 2248. https://doi.org/10.3390/pr11082248

Gusbakti, R., Ilyas, S., Lister, I. N. E., et al. (2022). Pengaruh konsumsi ekstrak buah naga merah terhadap kadar malondialdehid dan superoksida dismutase setelah latihan berat pada tikus (Rattus norvegicus). F1000Research, 10, 1061. https://doi.org/10.12688/f1000research.54254.3

Hofmann, E., Schwarz, A., Fink, J., Kamolz, L.-P., & Kotzbeck, P. (2023). Modelling the complexity of human skin in vitro. Biomedicines, 11(3), 794. https://doi.org/10.3390/biomedicines11030794

Hu, Q., Zhou, M., & Wei, S. (2018). Progress on the antimicrobial activity research of clove oil and eugenol in the food antisepsis field. Journal of Food Science, 83(6), 1476–1483.

Ighodaro, O. M., & Akinloye, O. A. (2018). First line defence antioxidants—superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54(4), 287–293.

Ivey, K. L., Jensen, M. K., Hodgson, J. M., et al. (2017). Association of flavonoid rich foods and flavonoids with risk of all cause mortality. British Journal of Nutrition, 117(10), 1470–1477.

Kauppila, T. E., Kauppila, J. H., & Larsson, N. G. (2017). Mammalian mitochondria and aging: An update. Cell Metabolism, 25(1), 57–71.

Kiki, M. J. (2023). In vitro antiviral potential, antioxidant, and chemical composition of clove (Syzygium aromaticum) essential oil. Molecules, 28(6), 2421. https://doi.org/10.3390/molecules28062421

Kristiningrum, N., & Cahyani, Y. N. (2015). Perbandingan kadar fenol total dan aktivitas antioksidan ekstrak metanol daun kopi robusta dan arabika. Jurnal e Pustaka Kesehatan.

Krutmann, J., Schikowski, T., Morita, A., & Berneburg, M. (2021). Environmentally induced (extrinsic) skin aging: Exposomal factors and underlying mechanisms. Journal of Investigative Dermatology, 141(4), 1096–1103.

Lee, S. E., & Park, Y. S. (2021). The emerging roles of antioxidant enzymes by dietary phytochemicals in vascular diseases. Life, 11(3), 199.

Lephart, E. D. (2018). Equol’s anti aging effects protect against environmental assaults by increasing skin antioxidant defense and ECM proteins while decreasing oxidative stress and inflammation. Cosmetics, 5(1), 16.

Lesmana, D., Andrianto, D., & Astuti, R. I. (2021). Antiaging properties of the ethanol fractions of clove (Syzygium aromaticum l.) bud and leaf at the cellular levels: Study in yeast Schizosaccharomyces pombe. Scientia Pharmaceutica, 89(4), 45.

Lesmana, D., Andrianto, D., & Astuti, R. I. (2021). Antiaging properties of the ethanol fractions of clove (Syzygium aromaticum l.) bud and leaf at the cellular levels: Study in yeast Schizosaccharomyces pombe. Scientia Pharmaceutica, 89(4), 45.

Mesa Arango, A. C., Flórez Muñoz, S. V., & Sanclemente, G. (2017). Mechanisms of skin aging. Iatreia, 30(2), 160–170.

Mohideen, K., Jeddy, N., Krithika, C., Faizee, S. H., Dhungel, S., & Ghosh, S. (2023). Assessment of glutathione peroxidase enzyme response and total antioxidant status in oral cancer—Systematic review and meta analysis. Cancer Reports, 6(8), e1842.

Moussa, Z., M. A. Judeh, Z., & A. Ahmed, S. (2019). Nonenzymatic exogenous and endogenous antioxidants. IntechOpen. https://doi.org/10.5772/intechopen.87778

Nikoui, V., Ostadhadi, S., Bakhtiarian, A., Abbasi Goujani, E., Habibian Dehkordi, S., Rezaei Roshan, M. & Giorgi, M. (2017). The anti inflammatory and antipyretic effects of clove oil in healthy dogs after surgery. PharmaNutrition, 5(2), 52–57.

O'Connor, C., Courtney, C., & Murphy, M. (2021). Shedding light on the myths of ultraviolet radiation in the COVID 19 pandemic. Clinical and Experimental Dermatology, 46(1), 187–188.

Papaccio, F., D′ Arino, A., Caputo, S., & Bellei, B. (2022). Focus on the contribution of oxidative stress in skin aging. Antioxidants, 11(6), 1121.

Pitojo, S. (2004). Benih buncis. Yogyakarta, Indonesia: Kanisius.

Prakash, A. (2021). Antioxidant activity [Report]. Surakarta, Indonesia: Medallion Laboratories Analytical Progress.

Razafimamonjison, G., Jahiel, M., Duclos, T., Ramanoelina, P., Fawbush, F., & Danthu, P. (2014). Bud, leaf and stem essential oil composition of Syzygium aromaticum from Madagascar, Indonesia and Zanzibar. International Journal of Basic and Applied Sciences, 3(3), 224.

Rusu, M. E., Fizeșan, I., Vlase, L., & Popa, D. S. (2022). Antioxidants in age related diseases and anti aging strategies. Antioxidants, 11(10), 1868.

Sugiyono. (2019). Metode penelitian kuantitatif, kualitatif dan R&D. Bandung, Indonesia: Penerbit Alfabeta.

Tang, M., Fang, R., Xue, J., Yang, K., & Lu, Y. (2022). Effects of catalase on growth performance, antioxidant capacity, intestinal morphology, and microbial composition in yellow broilers. Frontiers in Veterinary Science, 9, 802051. https://doi.org/10.3389/fvets.2022.802051

Toripah, S., Abidjulu, J., & Wehantouw, F. (2016). Aktivitas antioksidan dan kandungan total fenolik ekstrak daun kelor (Moringa oleifera L.). Jurnal Ilmiah Farmasi, 3(4), 2303–2493.

Wang, A. S., & Dreesen, O. (2018). Biomarkers of cellular senescence and skin aging. Frontiers in Genetics, 9, 389916.

Warraich, U. E., Hussain, F., & Kayani, H. U. R. (2020). Aging — oxidative stress, antioxidants and computational modeling. Heliyon, 6(5), e04107. https://doi.org/10.1016/j.heliyon.2020.e04107

WHO. (2017). WHO report on the global tobacco epidemic 2017: Monitoring tobacco use and prevention policies. Geneva, Switzerland: World Health Organization. Licence: CC BY-NC-SA 3.0 IGO.

Winarsih, H. (2017). Antioksidan alami & radikal bebas. Yogyakarta, Indonesia: Kanisius.

Wong, Q. Y. A., & Chew, F. T. (2021). Defining skin aging and its risk factors: A systematic review and meta analysis. Scientific Reports, 11(1), 22075.

Yousef, H., Alhajj, M., & Sharma, S. (2017). Anatomy, skin (integument), epidermis.

Zhang, S., & Duan, E. (2018). Fighting against skin aging: The way from bench to bedside. Cell Transplantation, 27(5), 729–738.




DOI: https://doi.org/10.51849/j-p3k.v6i2.621

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